TABLE 2.

Quantitation of leukemic cells in livers of singly versus doubly transgenic Mov13 mice^a

Litter	Mouse	Mov13	gag-SN (E)	%ROIb	Age (days)
1	1486	+	+	1.99
	1487	+	+	4.78
	1488	+	−	32.51
	1491	+	+	2.19	303
	1493	+	+	2.24
2	1713	+	−	1.47
	1418	+	+	1.73
	1719	+	−	57.69	237
	1720	+	−	2.88

^aOnly singly transgenic Mov13 mice that do not express the antiviral Gag-SN fusion protein exhibit significant infiltration by leukemic cells (mice 1488 and 1719). Livers from 15 mice (4 litters) resulting from Mov13/+ × gag-SN/+ and Mov14/Mov14 × gag-SN/+ crosses were analyzed for leukemic infiltrates by immunohistochemical staining with a monoclonal antibody against CD45 followed by image analysis. Animals included in the table were sacrificed and analyzed at 303 days of age (litter 1) and 237 days of age (litter 2). The presence of the Mov13 and/or gag-SN transgene of line E [gag-SN (E)] is indicated. The remaining six mice (two Mov14 litters, 179 days old) included in the study did not exhibit any infiltration above background (data not shown). Infiltration was quantified as described in Materials and Methods. 

^b%ROI, mean percent area scores of region of interest of liver staining positive for CD45.