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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Exp Gerontol. 2021 May 19;151:111416. doi: 10.1016/j.exger.2021.111416

Fig. 3.

Fig. 3.

Effects of oral senolytic agents on senescent cells, suprarenal aortic structure, and aortic wall gene expression. VVG staining shows elastin fragmentation with Ang II administration, but less fragmentation and increased elastin component with D + Q treatment (A). Smooth muscle cell marker aSM actin staining showed increased positivity with D + Q treatment (B). Macrophage staining revealed decreased CD68 positivity with D + Q treatment (C). Senescent cell markers HMGB-1 and GLB-1 were abundant in aortic tissue of aged mice treated with Ang II, but reduced with oral senolytics D + Q (D,E). mRNA expression of smooth muscle cell markers TAGLN, ACTA2, and CNN1 were increased in the presence of senolytics (F). mRNA expression of MMPs (MMP9, MMP2) were decreased in aortic tissue of mice given senolytics (G). Gene expression of inflammatory markers (H) and senescent cell markers (I) were decreased with senolytic treatment. Boxplots show the 25th and 75th percentiles (box), median (line), and whiskers defined by largest and smallest values within 1.5 times the interquartile range with individual points representing values beyond this range from N = 10–12 mice per treatment group for immunohistochemistry and 4–6 per group for gene expression data. * indicated significantly (P < 0.05) different from Ang II +, D + Q−.