FIG. 9.

(A) Analysis of numbers of CD3⁺ cells in the brain. Six different brain sections containing specimens from nontransgenic littermates or GFAP-HIV gp120 mice were immunostained for CD3 and analyzed by two different individuals. The average cell count was calculated, and statistical significance was determined using the Student t-test. The presence of CD3⁺ T cells in the olfactory bulb (∗, P < 0.07), neocortex (∗, P < 0.00007), and subcortex (∗, P < 0.002) was significantly increased compared with the wild-type mice. (B) Colocalization of infiltrating CD3⁺ lymphocytes and CXCL10/IP-10 RNA in the brain of GFAP-HIV gp120 transgenic mice. Sections from a wild-type control and a GFAP-HIV gp120 mouse were hybridized with the CXCL10/IP-10 antisense probe and immunostained for CD3 as described in Materials and Methods. Original magnification, ×450.