FIG. 1.

Schematic representation of the wild-type and chimeric GPs. Open boxes, BDV sequences. BDV p56 is processed via cleavage by the protease furin. A canonical furin recognition sequence (FS) is found at amino acids 245 to 249 of BDV p56. Striped boxes, hydrophobic sequences corresponding to the predicted signal peptide (SP) and the putative fusion domain of BDV p56; hatched box, long ectodomain of VSV G. Boxes representing the GS, TM domain, and CT of VSV G are indicated. In the chimeric constructs the CT and TM domain of VSV G were exchanged for the CT and TM domain of BDV p56. GGG, addition of three glycine residues at the junction between BDV p56 sequences and VSV G sequences in construct p56/GlyGS.