FIG. 3.

Incorporation of wild-type BDV p56 GP and BDV-VSV chimeric GPs into VSVΔG* particles. VSVΔG* particles complemented by transfection with the GPs indicated after each hyphen (lanes 2 to 6) or with empty control pCAGGS (lane 1) were partially purified by centrifugation through 20% sucrose and lysed in sample buffer. Proteins were separated by SDS-PAGE in 8 to 16% gels under reducing conditions and analyzed by Western blotting with an anti-p56 polyclonal rabbit serum (top). Vero cells mock infected (lane 7) or persistently infected with BDV (lane 8) were used as negative and positive controls, respectively. The same membrane was then stripped and reacted with an anti-VSV P rabbit serum (bottom).