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. 2024 Oct 1;24:1166. doi: 10.1186/s12903-024-04882-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Characterization of the extracellular matrix and decellularized extracellular matrix. (A) Conceptual framework depicting the experiment from ECM production to reseeding of gingival fibroblasts (GFs) on decellularized ECM. (B) Both ECM samples exhibited positive staining for TYPE I COLLAGEN and FIBRONECTIN. The intracellular cytoskeleton and nuclei were visualized using phalloidin and DAPI, respectively. No residual cells were observed, as indicated by negative actin and DAPI staining in the dECM