Theme 1	AOP networks and their applications
Charge Questions	• Definition of an AOP network • Identification of key considerations in designing and evaluating AOP networks, including appropriate experimental designs or structurual considerations for development based on anticipated application • Determine how AOPs could better capture changes in toxicity due to timing of exposure or biological changes over time (e.g., acute versus chronic toxicity, delayed toxicity, epigenetics, i.e., heritable changes that do not involve modifications to the DNA sequence, repeat exposures, feedback loops and compensatory mechanisms) • Define for which species the AOP network is most applicable to; • Describe how AOP networks can be used to understand complex chemical mixtures
Theme 2	Quantitative AOPs (qAOPs) and their applications
Charge Questions	• Define exactly what consistutes a qAOP • Identify experimental design considerations appropriate for developing models for and applying qAOPs, including what level of detail is necessary based on the application • Determine how uncertainty should be captured for transparency when developing models to support qAOPs • Describe how qAOPs can be used in conjunction with exposure information, such as absorption, distributuion, metabolism and elimination data in risk assessment
Theme 3	Regulatory use of the AOP framework
Charge Questions	• Describe to what regulatory scenarios AOPs could be applied, including consideration of geographic location and regulatory authority similarities and differences • Consider how one would evaluate fit-for-purpose of an AOP • Determine how regulatory needs may inform AOP development
Theme 4	Expanding awareness of, involvement in, and acceptance of AOPs to support aspects of predictive toxicology and regulatory decision-making
Charge Questions	• How can education and outreach related to the AOP framework be effectively and efficiently improved • Encourage/incentivize coordination and development of AOPs • Increase recognition of AOPs, and enhanced uptake across stakeholder communities • Facilitate adoption of the AOP framework for regulatory application