FIG. 2.

SV infection induces apoptosis and lysis of cortical neurons. Cortical cells were infected at an MOI of 5 with SV-GFP. (A) Digital images of Hoescht-stained nuclei of uninfected (a) and infected (b and c) cells. The nuclei of infected cells appear condensed and fragmented. (B) Digital images of SV-GFP-infected cortical cells were acquired every 5 min. The arrow indicates an SV-infected cortical neuron that lysed during the time interval shown. GFP, a small cytoplasmic protein, is released from the cell after loss of plasma membrane integrity. (C) Digital images for PI were acquired every 25 min. PI (5 μg/ml) was included in the imaging medium. The cell indicated by the arrow is the same cell shown in panel B and became PI positive and GFP negative in the 25-min interval shown. The arrowheads indicate cells adjacent to a lysed cell which became PI positive.