Table 1.

Primers designed for this study and the reference sequences for designing the primers.

Genetic Marker	Primers Designed	Annealing Temperature (°C)	PCR Amplicons Size (bp)	Accession No. of DNA Sequences Used for Primer Design
mtCOI	Hs-COX1_Fwd	50	386	MH571871, MH571870, NC_029838, NC_029839, OR177661, KY368775, KT900946, KT764937
	5′GAT TTT GCC TGC TTT TGG TAT 3′
	Hs-COX1_REV
	5′CTC ACC ACA TAA TAA GTA TCA 3′
Nuclear rRNA ITS1	ITS1-Heterakis-F	60	563	JX845277, JX845278, MF319969, MH215352, EF464551, EF464554, EF464553, KP776414, KU575094
	5′GAG CCR CGT AGT GTA CTA CAA 3′
	ITS1-Heterakis-R
	5′TCG ACC CTC AGC CAG ACG TG 3′