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Determination of the IC50 of 2 for P. aeruginosa PBP3 using a competitive assay. Bocillin™, a fluorescent substrate of PBP3 was reacted with enzyme that had been pre-incubated with increasing concentrations of 2. An IC50 was calculated as the concentration of 2 required to reduce the fluorescence intensity of the Bocillin™-labeled protein by 50%.
