Table 6.
Susceptibility of 2 to β-Lactamase hydrolysisa
| Metallo-β-lactamase | kcat (s−1) | Km (μM) | kcat/Km (μM−1s−1) |
|---|---|---|---|
|
| |||
| NDM-1 | 35 ± 4 | 1808 ± 200 | 0.020 ± 0.002 |
| VIM-2 | 54 ± 6 | 4400 ± 450 | 0.012 ± 0.001 |
| IMP-1 | 5 ± 1 | 1490 ± 300 | 0.003 ± 0.001 |
| KPC-2 | NM | NM | NM |
| ADC-7 | NM | NM | NM |
| OXA-23 | NM | NM | NM |
a
Steady-state reactions of 2 were monitored using purified enzymes: KPC-2, ADC-7, OXA-23, NDM-1, VIM-2, and IMP-1. Kinetic parameters provided were determined from double reciprocal plots (Supplemental Figure 2, page 44). Measurable reaction was not detected for KPC-2, ADC-7, or OXA-23 using 200 nM enzyme concentration and 100 μM 2. NM = not measureable.