FIG. 3.

Immune responses primed by IHGI. (A and B) CD4⁺ and CD8⁺ cell-mediated T-cell responses in the periphery (A) and in the liver (B) were assayed as described in Materials and Methods. CD8⁺ responses tested against a panel of VV-infected autologous B-LCLs were scored positive when percent specific lysis at the two highest E:T ratios were greater than or equal to the percent lysis of VVwt-infected targets plus 10 (see text for specifics). HCV-specific CD8⁺ CTL clones were isolated from liver at week 6 (★). CD4⁺ responses were tested against the following recombinant HCV 1a proteins: C22-3 (core; blue), E1-E2 (orange), C200 (NS3/4; purple), and NS5 (green). An SI of 3.0 or greater was considered positive. (C) Antibody responses against C22-3 (core; blue line), C33c (NS3; red line), C25 (core plus NS3/4; yellow line), C100 (NS3/4; purple line), and NS5 (green line). Signal-to-cutoff ratios were, respectively, 0.109, 0.098, 0.174, 0.142, and 0.094 optical density (OD) units. (D) Antibody (Ab) responses against E2 (turquoise line), E1-E2 (orange line), and H77 HVR1 variant N/S (aa 384 to 410; black line/broken black line). Signal-to-cutoff ratios were 0.097, 0.126, and 0.139/0.121 optical density units, respectively.