Fig. 6.

Simulated structure of Ab2c2 MTG. a A ribbon model (purple) of the overall structure of D3C/G283C MTG molecules B. The mutated residues C3 and C283 and three additional mutation sites (S101, G157, and G250) in Ab2c2 MTG are shown with sphere models. Labels P-1 and P-2 indicate two protrusions which construct the active site cleft. b, c, and (d) Superimposed stick models (stereo view) of the simulated structure of Ab2c2 MTG (blue) and the crystal structure of D3C/G283C MTG (purple), around the mutated residues 101, 157, and 250. Putative hydrogen bonds and salt bridge observed in the simulated structure of Ab2c2 MTG are represented with green lines accompanied by the atomic distances (Å unit)