Table 1.

Residual activity after heat shock, TG secretion and relative activity of wild-type, D3C/G283C and mutants and amino acid substitutions due to mutation

Amino Acid Substitution	Residual Activity at 65 °C	TG secretion	Relative Activity at 37 °C
	Ratio	mg/mL	Ratio
Wild-type MTG	0.038	0.59	1.00
D3C/G283C	0.064	0.37	1.06
S101P	0.541	0.32	0.96
V112I/G250R	0.495	0.35	0.98
A83T	0.490	0.31	0.60
N92S/G250R	0.421	0.36	1.17
T53S/G157S	0.411	0.38	1.01
G157A	0.409	0.35	0.97
G250A	0.391	0.35	1.27
G250E	0.226	0.30	0.72
T53S	0.201	0.33	1.52
N92D/G250R	0.188	0.36	1.33
R79S	0.144	0.36	0.68
A267V	0.130	0.30	1.01
N297K	0.104	0.30	0.92

The ratio of residual activity was calculated between non-incubated supernatant and heat-shocked supernatant at 65°C for 10 min. Secretion of protein was determined by analytical reverse-phase HPLC. The ratio of relative activity was calculated between mutants and wild-type