Table 4.

Measurement of the T₅₀ value and kinetic constant at 37 °C of wild-type, D3C/G283C and Ab2c2 MTG

Enzyme	T50	∆T50	Vmax	Km	kcat	kcat/Km
	°C		μmol/min/mg	mM	s−1	M−1 s−1
Wild-type MTG	57.2	0	0.53 ± 0.07	1.07 ± 0.40	0.34 ± 0.045	332 ± 80
D3C/G283C	66.1	+ 8.9	0.49 ± 0.02	0.82 ± 0.14	0.31 ± 0.011	387 ± 55
Ab2c2	67.3	+ 10.1	0.75 ± 0.07	0.95 ± 0.44	0.47 ± 0.043	590 ± 143

T₅₀ is defined as the temperature of incubation at which 50% of the initial activity of the enzyme solution remained. ∆T₅₀ values represent the difference between wild-type MTG and mutant enzymes

Assay of kinetic constants were carried out as described in Materials and Methods. Data were expressed as mean ± SD (n = 3)