Table 3.
Additional non-synonymous SNPs identified in insecticide resistance genes vgsc, gste2, rdl and ace1. Includes the number of mosquitoes and their phenotype per SNP. SNPs with an allele frequency over 5% in the sample population are highlighted in bold. Ref = reference alt = alternate.
| Chrom | Gene | Position | Ref | Alt | Amino acid Change | Resistant mosquitoes (n = 23) | Susceptible mosquitoes (n = 10) | Control mosquitoes (n = 9) | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Heterozygous | Homozygous Alternate | Heterozygous | Homozygous Alternate | Heterozygous | Homozygous Alternate | ||||||
| 2 L | vgsc | 2,431,232 | G | A | G1991E | 1 | 0 | 0 | 0 | 0 | 0 |
| vgsc | 2,431,330 | A | T | T2024S | 1 | 0 | 0 | 0 | 0 | 0 | |
| vgsc | 2,431,394 | C | T | P2045L | 0 | 0 | 0 | 0 | 1 | 0 | |
| 3R | gste2 | 28,597,772 | C | A | K215N | 0 | 1 | 0 | 0 | 0 | 0 |
| gste2 | 28,597,891 | C | T | E176K | 2 | 0 | 1 | 0 | 1 | 0 | |
| gste2 | 28,597,956 | G | C | T154S | 13 | 4 | 5 | 1 | 5 | 1 | |
| gste2 | 28,598,041 | T | A | I126F | 3 | 0 | 1 | 0 | 1 | 0 | |
| gste2 | 28,598,505 | C | T | G26S | 2 | 0 | 1 | 0 | 0 | 0 | |
| gste2 | 28,598,568 | C | G | V5L | 1 | 0 | 0 | 0 | 1 | 0 | |
| gste2 | 28,598,573 | T | A | N3I | 1 | 0 | 0 | 0 | 1 | 0 | |
| 2 L | rdl | 25,382,837 | G | A | V63I | 1 | 0 | 0 | 0 | 0 | 0 |
| rdl | 25,433,554 | C | T | P473S | 1 | 0 | 0 | 0 | 0 | 0 | |
| 2R | ace1 | 3,489,253 | G | A | G14E | 1 | 0 | 0 | 0 | 0 | 0 |
| ace1 | 3,489,358 | C | G | A49G | 1 | 0 | 0 | 0 | 0 | 0 | |
| ace1 | 3,489,391 | C | T | A60V | 0 | 0 | 1 | 0 | 0 | 0 | |
| ace1 | 3,489,405 | G | T | A65S | 14 | 5 | 7 | 2 | 4 | 1 | |
| ace1 | 3,489,475 | C | T | S88L | 0 | 0 | 0 | 0 | 1 | 0 | |
| ace1 | 3,491,883 | C | T | T216I | 1 | 0 | 0 | 0 | 0 | 0 | |
| ace1 | 3,493,410 | G | A | S648N | 0 | 0 | 0 | 0 | 1 | 0 | |
| ace1 | 3,493,714 | G | T | G1991E | 0 | 0 | 1 | 0 | 0 | 0 | |