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. 2024 Oct 1;9:180. doi: 10.1038/s41541-024-00979-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — A Flow cytometry analysis of exogenous hGPRC5D expressed in 293T or B16-F10 cells transfected with Nano-hGPRC5D or pUltraHot-hGPRC5D. B, C Flow cytometry and western blot analysis of hGPRC5D expression in mouse cells stably expressing hGPRC5D. D Western blot analysis of hGPRC5D expression in the muscle of C57/BL6J mice immunized with Nano-EGFP or Nano-hGPRC5D.