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. 2024 Oct 1;15:8482. doi: 10.1038/s41467-024-52752-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — A Structure of the gp53 locus in DEV and DEV Δ53 phages and the pD53 plasmid. crRNA HR, region targeted by cr-RNA53 expressed by pCas3-09; HR1 and HR2, homology regions cloned in pCas3-09 plasmid; gp53_R, cr-RNA53 resistant gp53 allele cloned in pD53. B Outline of DEV mutagenesis. Infection with DEV of PAO1 carrying pCas3-09 and pD53 produces a genetically mixed phage progeny with wt (in blue) and Δ53 (in green) virions. Unlike gp53⁺ DEV, Δgp53 mutants grow on PAO1 carrying pD53 (pD53) but not on PAO1 containing the empty vector pGM931 (vector). Source data are provided as a Source Data file. C DEV Δgp53 growth on mutants with LPS defects. Serial dilutions (x 10) of DEV or Δgp53 were replicated on PAO1 and the indicated PAO1 mutants with defective LPS. On the right is the structure of PAO1 LPS (c, capped) and the LPS portions present in the mutant LPS variants. tc, truncated core; u + 1, uncapped LPS + one O-antigen repeat. Source data are provided as a Source Data file.