Fig. 2. Sample location selectivity is highest in the MOs during the sample phase of the DMNTP task.

a Example raster plot from an MOs neuron increasing its firing rate around the sample port poke on all correct trials. b Z-scored heat maps of all neurons recorded from each region around the sample poke (white dashed line) sorted by each neuron’s mean Z-scored firing rate 500 ms before the sample poke. c The mean Z-scored pseudopopulation firing rate is higher in MOs leading up to the sample poke compared to the other regions. Lighter shaded areas above and below the solid lines represent the standard error of the mean at each time point. d The percent of neurons significantly increasing or decreasing their firing rate from baseline (ITI) in at least two 100 ms bins is also higher in the MOs. e Example sample location-sorted raster plots of two MOs neurons exhibiting transient selective firing for the right or left sample location, respectively. Red line separates left from right sample trials. f MOs contains the highest percentage of neurons selective for either retrospective sample location (Left and Right combined) in a ~1 s window around the sample poke. g Sample location decoding accuracy of a linear support vector machine trained and tested on every combination of 200 ms time bins from the sample window. White lines indicate the exact time the mouse poked the sample port. Grey area represents non-significant decoder accuracy compared to a trial identity-shuffled control. In panels c, d, and f, double-colored straight lines represent statistically significant differences (p-value < .05) between the two respective subregions in that time bin, after correcting for both false discovery rate and family-wise error rate.