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. 2024 Aug 14;43(19):4324–4355. doi: 10.1038/s44318-024-00194-2

Figure EV4. Cryo-EM analysis of the nucleosome core particle in complex with APC3 loop177-446.

Figure EV4

(AC) Workflow showing a representative micrograph, the cryo-EM data collection parameters (A) and the single-particle analysis pipeline for the nucleosome core particle with the APC3 loop177-446 (B, C). N. of particles at each classification step is indicated. (D) Fourier shell correlation (FSC) curves, angular distribution plot and plot of the directional FSC that represent a measure of directional resolution anisotropy for all the reconstructions are shown. Directional FSC and sphericity determination was performed with the 3DFSC software (Tan et al, 2017).