Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Aug 14;43(19):4324–4355. doi: 10.1038/s44318-024-00194-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A) EMSA of the APC/C-CDC20-Cyclin B1^NTD and NCP147. N = 3 independent experiments. (B) Representation of the heteromeric crosslinks found between the APC3 loop and the acidic patch of the NCP. (C) A sequence alignment of the C-terminus of the human APC3 loop with homologous sequences in other species. APC3 K390, which crosslinks with the acidic patch, is marked with a green arrow. Previously identified phosphorylation sites are marked with black arrows. (D) EMSA of the APC3 loop WT and charge-substitution 3R3E mutant with the NCP147. N = 3 independent experiments. Bands corresponding to free nucleosome and to the APC3:nucleosome complex are marked. Non-specific binding events are indicated with an asterisk. (E) EMSA of the APC3 loop WT including LANA peptide. N = 3 independent experiments. (F) A close-up view of the APC3 loop RRSSR motif bound to the acidic patch of the NCP. The APC3 loop is shown as balls and sticks in dark purple, residues 375–381 can be seen. H2A and H2B are shown in the cartoon representation. Dashed lines indicate hydrogen bonds between the APC3 loop and the acidic patch and surrounding residues. R380 establishes hydrogen bonds with E61, D90, and E92 of the acidic patch, and R376 with E56. (G) Cryo-EM density of the APC3 loop and surrounding H2A/H2B residues. Source data are available online for this figure.