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. 2024 Aug 14;43(19):4324–4355. doi: 10.1038/s44318-024-00194-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) The elution profile of APC/C^CDC20 with Cyclin B1^WT (cyan) or Cyclin B1^Δ9 (magenta) on size-exclusion chromatography. Graph representative of N = 3 independent experiments. (B) Representative immunoblot of the size-exclusion chromatography is shown in (A). (C, D) Left: Maximum projections of confocal images representative of RPE-1 Cyclin B1-mEmerald^+/+ cells ectopically expressing the indicated variant of Cyclin B1-mScarlet. The scale bar corresponds to 10 μm. Middle: Graphs representing the pixel-by-pixel fluorescence intensity over a line going from centrosome to centrosome of RPE-1 Cyclin B1-mEmerald^+/+ (Cyan) cells ectopically expressing the indicated variant of Cyclin B1-mScarlet (Magenta). Grey indicates siR-DNA staining: n ≥ 38 cells per condition, N ≥ 3 independent experiments. Mean ± standard deviation are plotted. Right: Cyclin B1 degradation graph representing the fluorescence intensity of Cyclin B1 over time of RPE-1 Cyclin B1-mEmerald^+/+ (Cyan) cells ectopically expressing the indicated variant of Cyclin B1-mScarlet (Magenta): n ≥ 15 cells per condition, N ≥ 3 independent experiments. Mean ± standard deviation are plotted. Source data are available online for this figure.