FIG. 1.

Ad infection inhibits TRAIL-induced apoptosis as assessed by nuclear morphology. A549 cells were mock infected or infected with WT Ad (rec700) (100 PFU/cell). At 19 h p.i., the cells were treated with leucine zipper TRAIL (20 ng/ml) plus CHX (25 μg/ml). After 26 h, the cells were fixed in paraformaldehyde and then permeabilized with methanol containing DAPI. The cells were immunostained for the Ad-encoded DBP (72). (A) Two fields of mock-infected DAPI-stained nuclei. All the nuclei shown are apoptotic. (B) The same field of WT Ad-infected cells immunostained for DBP (left) and stained with DAPI (right). All the infected cells shown, as indicated by the speckled staining for DBP in the nucleus, had nonapoptotic nuclei.