FIG. 5.

RID mediates internalization of cell surface TRAIL-R1 into putative endosomes and lysosomes, where TRAIL-R1 is degraded. (A to F) Cells were mock infected or infected with WT Ad (rec700) or a RID⁻ 14.7K⁻ mutant (dl309). At 6 and 23 h p.i., the cells were fixed in paraformaldehyde and then permeabilized with methanol. The cells were immunostained for TRAIL-R1 using the M271 MAb (Immunex) and FITC-conjugated goat anti-mouse IgG. For the 6- and 23-h time points, 400 and 50 PFU of virus/cell, respectively, were used. (G to I) Same as for panels D to F, except the cells were treated with Baf (0.1 μM) beginning at 6 h p.i.