FIG. 7.

Infection with replication-defective E1-minus Ad vectors expressing RID removes TRAIL-R1 but not TfnR from the cell surface. HeLa cells were mock infected or infected with 5 to 20 PFU of rec700 or Ad vectors/cell. At 23 h p.i., unfixed cells were stained with MAbs for TRAIL-R1 or TfnR and assayed by flow cytometry. Ad/E3 encodes and expresses all E3 proteins except ADP (E3-12.5K, E3-6.7K, E3-gp19K, RIDα, RIDβ, and E3-14.7K). Ad/E3, Ad/RID/14.7K, Ad/RID, and Ad/14.7K express only the E3 proteins indicated.