TABLE 1.
Viruses used in this studya
| Virusb | RIDα | RIDβ | E3-14.7K | E1B-19K |
|---|---|---|---|---|
| rec700 | + | + | + | + |
| Ad5 | + | + | + | + |
| dl111 | − | − | − | − |
| dl309 | − | − | − | + |
| lp5 | + | + | + | − |
| dl250 | + | + | + | − |
| dl764 | + | − | + | + |
| dl799 | − | − | + | + |
| dl758 | + | + | − | + |
| dl762 | + | + | − | + |
| pm760 | ++ | ++ | ± | + |
| dl7000 | − | − | + | + |
| dl701 | + | + | + | + |
| dl704 | + | + | + | + |
| dl731 | + | + | + | + |
| pm734.1 | + | + | + | + |
| Ad/E3 | + | + | + | − |
| Ad/RID/14.7K | + | + | + | − |
| Ad/RID | + | + | − | − |
| Ad/14.7K | − | − | + | − |
All mutants except Ad/E3, Ad/RID/14.7K, Ad/RID, and Ad/14.7K are fully competent for replication in cultured cells. Ad/E3 is an E1− replication-defective Ad vector expressing all E3 Ad proteins except ADP from the CMV promoter. E3 c-encoded proteins are named RIDα, RIDβ, 14.7K, gp19K, 6.7K, 12.5K, and ADP. RIDα and RIDβ function together as the protein complex named RID. Ad/RID/14.7K is an E1− vector expressing only RID and 14.7K. Ad/RID is an E1− vector expressing only RID. Ad/14.7K is an E1− vector expressing only 14.7K. +, expressed; −, not expressed; ++, overexpressed; ±, underexpressed.
rec700 is an Ad5-Ad2-Ad5 recombinant and is the WT parent for mutants with 700 or 7000 numbers. Ad5 is the WT parent for dl111 and dl309. lp5 and dl250 are derived from Ad2, which is very closely related to Ad5 and rec700. dl701 lacks the gene for the E3-6.7K protein. dl704 lacks the gene for the E3-gp19K protein. dl731 lacks the gene for the E3-12.5K protein. pm734.1 lacks the gene for ADP (previously named E3-11.6K).