Fig. 3.

Coherence at the gamma band in the OB^M/T→EC circuit monitors the retrieval of cognitive memory. (A) Schematic and histology of AAV-DIO-eNpHR3.0-mCherry or AAV-DIO-mCherry injection (as control) into the ventral OB and optical cannula implantation above the bilateral EC and single-electrode implantation in the OB and bilateral EC. DAPI was used as a nuclear marker. (B) Schematic of olfactory-related working memory test and quantification of latency to zone A or B and the total time in zone A or B during the training session (n = 9 in each group). (C) Quantification of latency to zone A and the total time in zone A during the test session (n = 9 in each group). (D) Relative power spectrum of LFPs recorded in the left EC around the onset of exploring zone A (n = 6 in each group). (E) Power spectrum of LFPs (3 to 95 Hz) in the EC during exploring zone A in the test session (n = 6 in each group). (F and G) Heatmap and quantification of OB-EC coherence of LFPs in control and eNpHR3.0 mice (n = 6 in each group). (H) Pearson’s correlation analysis of latency to zone A and total time in zone A during the test session versus OB-EC coherence at the gamma band. Data are presented as mean ± SEM; ^*P < 0.05, ^**P < 0.01, ^#P < 0.05. Two-way ANOVA for (B) and (H). Unpaired 2-tailed Student’s t test for (C) and (D).