Figure 1.

Chemotherapy induces weight stagnation, bile acid malabsorption, and altered fecal bacteriome composition. (a) Body mass changes from baseline. (b) Changes in 48 hour food intake from baseline. (c) Average feed efficiency during the study period. (dD) average light cycle, dark cycle, and total locomotion changes from baseline. (e) Average light cycle, dark cycle, and total body temperature changes from baseline. (f) Fecal wet mass changes from baseline. (g) Fecal total BA excretion throughout the study period. (h) Fecal primary BA excretion throughout the study period. (i) Fecal TMCA excretion throughout the study period. (j) Heat tree displaying differences in fecal 16S rRNA gene amplicon profiles in Chemo vs vehicle the day following the final dose. Nodes display log2FoldChange and edges display number of amplicon sequence variants composing each node. Baseline measurements are the average of two days prior to treatment. (a-c) N = 18 (vehicle n = 10, chemo n = 8), (d-e) N = 16 (vehicle n = 8, chemo n = 8), (f-j) N ≤ 18 (vehicle n = 8-10, chemo n = 5-8), * p < 0.05.