Fig. 2.

Over-expression of circSSR1 inhibited vascular smooth muscle cell pyroptosis. A, Over-expression of circSSR1 reduce reactive oxygen species probes (DCFH-DA, green) staining in various groups (n = 6). B, Endoplasmic reticulum stress (ERS) related protein ATF6, IRE1 and GRP78 expression. C, LDH cytotoxicity assay kit (LDH) was used to detect LDH release (n = 6). D, Western blot to demonstrate pyroptosis related protein NLRP3, GSDMD and Caspase-1 expression (n = 5). E, Fluorescence staining of IL-1β in PASMCs under hypoxia (n = 6). F, Over-expression of circSSR1 plasmid decreased PI positive cells (n = 5). Scale bars, 50 μm. All values are presented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. NOR, normoxia and HYP, hypoxia. NC: negative control