Fig. 3.

Added endoplasmic reticulum stress (ERS) 4-PBA can restrain pyroptosis caused by hypoxia. A, Western blot indicated adding 4-PBA can inhibit NLRP3, GSDMD and Caspase 1 expression during hypoxia (n = 5–6). B, The positive cells were demonstrated by PI staining (n = 3). C, LDH release assay detected the content of lactate dehydrogenase (n = 6). D, Immunofluorescence showed inhibitor of ERS reduce IL-1β expression in the hypoxia (n = 6). E, Fluorescent probe DCFH-DA detected ROS release of cells treated with 4-PBA (n = 6). Scale bars, 50 μm. All values are presented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. NOR, normoxia and HYP, hypoxia. NC: negative control