Fig. 5.

CircSSR1 regulate expression of SSR1 by bonding to YTHDF1. A, catRAPID website was used to predict YTHDF1 bound to circSSR1 and cytoscape software drawing interaction network diagram. B, The heatmap demonstrated that circSSR1 can interact with YTHDF1 by catRAPID. C, HNADOCK Server predicted and visualized the circSSR1 and YTHDF1. D, RNA immunoprecipitation (RIP) experiments showed that YTHDF1 can combine with circSSR1 rather than SSR1 mRNA (n = 3). E, CircSSR1 and YTHDF1 can co-localization in the cytoplasm of PASMCs. F, Fast silver stain kit and RNA pull-down kit were used to indicated circSSR1 bound to YTHDF1. G, SRAMP website predicted there was three m6A site on SSR1 mRNA. H-I, RIP experiment showed m6A is located on SSR1 mRNA and YTHDF1 can recognize m6A site of SSR1 to promote SSR1 translation (n = 3–4). Scale bars, 50 μm. All values are presented as the mean ± SEM. **p<0.01, ***p<0.001. NOR, normoxia and HYP, hypoxia. NC: negative control