FIG. 2.

Identification and kinetics of expression of the PrV UL37 protein. RK13 cells were infected at an MOI of 10 with PrV-Ka (lanes 1 to 6) or PrV-ΔUL37 (lanes 7 to 12) and harvested at 1 (lanes 1 and 7), 3 (lanes 2 and 8), 5 (lanes 3 and 9), 7 (lanes 4 and 10), 9 (lanes 5 and 11), or 24 (lanes 6 and 12) h after infection. Lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and, after Western blotting, incubated with polyclonal sera against the UL37 protein (A), the UL34 protein (B), and the UL49 protein (C) or a monoclonal antibody against gC (D). Lanes 13, purified PrV-Ka virions. Lanes 14, purified PrV-ΔUL37 virions.