Figure 4.

Recombinant CNPY2 increases the survival of primary cortical neurons after ER stress. Neurons were prepared from brain cortices of E18 old rats and grown in culture for 7 days as described in Methods. Neurons (0.25 × 10⁶ cells) in 96 well plates were then treated with 2.5 μg/mL tunicamycin (Tun) for 24 h in the absence or presence of 100 ng/mL recombinant CNPY2. For the experiment, eight wells were used for each treatment that was done in triplicates. Cell viability was determined by MTT assay as described in Methods. Tun decreased cell viability of the neurons and this was counteracted by the addition of CNPY2. Values are means ± SD, n = 4. ^**p < 0.01 for T vs. C, and for CNPY2 + T vs. T.