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. 2024 Oct 2;10(40):eadp5491. doi: 10.1126/sciadv.adp5491

Fig. 4. PP2A-B55 regulates NEXT complex function through binding RBM7.

Fig. 4.

(A) Stable HeLa cell lines expressing the indicated constructs and RNA levels of indicated NEXT substrates measured by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Quantifications of data from three biological replicates. Error bars show mean and SD. (B) IP of RBM7 constructs and monitoring binding to B55. (C) MS comparison of RBM7 WT and R143A with NEXT components highlighted in blue. (D) Binding of RBM7 S136A and S136D to PP2A-B55 and table of affinities measured of RBM7 peptides measured by SPR. Quantifications of data from three biological replicates. Error bars show mean and SD. (E) Endogenous RBM7 was tagged with dTAG, allowing the rapid removal of RBM7; cells were complemented with the indicated RBM7 variants; and the indicated RNAs were quantified by RT-qPCR. Quantifications of data from two biological replicates and the average are shown.