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. 2024 Oct 2;10(40):eadp8266. doi: 10.1126/sciadv.adp8266

Fig. 4. NOD1 activation decreases PLIN5 expression to regulate the FAO and immunostimulatory function in TAMs.

Fig. 4.

(A) Heatmap of representative FFAs and acylcarnitines in TAMs treated with either vehicle or C12-iE-DAP (400 ng/ml) for 24 hours. (B and C) β-Oxidation rate and FFA levels were measured in TAMs with different treatments. (D) Flow cytometric analysis of IFN-γ+ OT-I CD8+ T cells after cocultured with TAMs that were transfected with either control or PLIN5 overexpression plasmids, followed by treatment with either vehicle or C12-iE-DAP (400 ng/ml), and then pulsed with SIINFEKL (OVA257-264). (E) Flow cytometric analysis of IFN-γ+ CD8+ T cells after cocultured with TAMs transfected with either control or PLIN5 overexpression plasmids, followed by treatment with either vehicle or C12-iE-DAP (400 ng/ml) for 24 hours. (F) Flow cytometry analysis of CFSE-labeled CD8+ T cell proliferation after cocultured with TAMs transfected with either control or PLIN5 overexpression plasmids, followed by treatment with either vehicle or C12-iE-DAP (400 ng/ml) for 24 hours. (G) Schematic diagram depicting the regulatory role of NOD1 on TAMs. Statistical analysis was performed using the Student’s t test. Data were presented as mean with SD. *P < 0.05, **P < 0.01, ***P < 0.001. LD, lipid droplet; OE, overexpression.