(A) Purification of ssD-BP from HeLa cells. SDS-polyacrylamide gel electrophoretic pattern of purified ssD-BP. Lane 1, protein molecular size markers; lane 2, WCE of HeLa S3 cells (20 μg of total protein); lane 3, Oct2A factor, included as a positive control, purified according to the standard protocol using the protein purification kit supplied by the vendor (Boehringer Mannheim); lane 4, purified ssD-BP. The arrow indicates the ≈52-kDa ssD-BP. (B) Deduced amino acid sequence of the human FKBP52 protein. The boldface underlined amino acids represent the identified homology between ssD-BP and FKBP52 (GenBank accession no. M88279).