FIG. 3.

In vitro phosphorylation of purified FKBP52 protein by CK II and EGFR-PTK. CK II was incubated in the absence (−; lane 1) or presence (+; lane 2) of 1 μg of FKBP52. Similarly, EGFR-PTK was incubated in the absence (lane 3) or presence (lane 4) of FKBP52 as described in Materials and Methods. The arrow indicates the phosphorylated FKBP52 protein, and the arrowheads denote the autophosphorylated CK II and EGFR-PTK proteins.