FIG. 7.

Electrophoretic mobility shift assays for the AAV D(−) sequence interaction with FKBP52 in mock-transfected NIH 3T3, 293, and HeLa cells (lanes 2, 4, and 6) or cells stably transfected with the human FKBP52 expression plasmid (lanes 3, 5, and 7). These assays were carried out as described in the legend to Fig. 4. The tyrosine-phosphorylated form of the FKBP52 protein is denoted by the arrow, and the serine- or threonine-phosphorylated form is denoted by the arrowhead. +, present; −, absent.