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. 2024 Jul 15;15(5):1772–1784. doi: 10.1002/jcsm.13529

Figure 3.

Figure 3

Acute loss of p53 induces a fasting‐like circadian gene expression signature in muscle. (A) Heatmap of circadian gene transcripts altered (P < 0.05; presented as Z‐scores) by acute Trp53 deletion in quadriceps in fed and fasted conditions from RNAseq analysis comparing control (LOX) and p53MKO (KO) mice under fed and 16‐h fasted conditions (n = 3–5). (B) 3D principal component analysis (PCA) representation and (C) hierarchical clustering analysis of fed control (white/black circles), fed p53MKO (light blue circles), fasted control (grey circles), and fasted p53MKO (dark blue circles) mice based on circadian‐related gene transcripts. (D) mRNA expression of Per1, Per2, Cry2, Cry1, Nr1d1, and Ppp1r3c in quadriceps of p53MKO mice in fed (LOX: White bars; p53MKO blue bars) and 16‐h fasted (LOX: Grey bars; p53MKO: Dark blue bars) conditions. Results presented as mean ± SEM; data in (D) were compared by two‐way ANOVA (n = 8–11); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.