Figure 7.

Anaerobic glucose breakdown occurs in response to elevated glucose mobilization following Trp53 inactivation. (A) Intramuscular glycogen levels, measured by a biochemical assay, in quadriceps muscle from control (LOX) and p53MKO (KO) mice during fed conditions (LOX: white bars; p53MKO: yellow bars) and following 4 h of food withdrawal (4 pm to 8 pm; LOX: Grey bars; p53MKO: orange bars) in mice directly after an abbreviated course of short‐term (3‐day) tamoxifen administration. (B) Pyruvate dehydrogenase (PDH) activity in quadriceps of control and p53MKO mice under fed and fasted conditions after short‐term tamoxifen. (C) Representative immunodetections and (D) quantifications of phosphorylated PDH (p‐PDH at S232), total PDH (t‐PDH), pyruvate dehydrogenase kinase isoforms (PDK1, PDK2, and PDK4) in quadriceps muscle under fed and fasted conditions and normalized to total corresponding protein (for p‐PDH) and total protein (TP) loading (conditions as described in panel A). (E) Plasma glucose, alanine, and lactate measured in plasma in fed and fast conditions in mice directly after an abbreviated course of short‐term (3‐day) tamoxifen administration. (F) Plasma glucose, alanine, and lactate measured in plasma of LOX and p53MKO mice during fed conditions (LOX: white bars; p53MKO: light blue bars) and following 16 h of food withdrawal (LOX: grey bars; p53MKO: dark blue bars) after receiving the full course of 5‐day tamoxifen and 2‐day recovery before organ collection. Results presented as mean ± SEM; data were compared by two‐way ANOVA (A–E: N = 3–5; F: N = 8–10); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.