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LC-mass spectroscopy of trypsin-digested VP22 illustrating residues 23 to 42 of the peptide sequence ENSLYDYESGSDDHVYEELR, which contains tyrosines 27, 29, and 38. A shift in mass from 2,420 to 2,500 m/z correlates with the phosphorylation of one of the three tyrosines in the peptide. amu, atomic mass units.
