FIG. 4.
VP22 is still tyrosine phosphorylated in vY38F mutant virus. VP22 and two degradation peptides are indicated by arrows. The N-terminal peptide (N-term) has a molecular mass of 22 kDa, and the C-terminal peptide has a molecular mass of 14 kDa. The purified BHV-1 and vY38F mutant viruses were analyzed by immunoblotting and probed with anti-VP22 antibody (A), anti-C-terminal-peptide antibody 114235–258 (B), or polyclonal antiphosphotyrosine antibody (C). The two lanes of vY38F represent two isolates. Note that the N terminus was phosphorylated in wild-type virus but that the C terminus was phosphorylated in vY38F. The ∼60- and 50-kDa bands observed in panel B may represent multimers of VP22 and the C-terminal peptide or nonspecific bands. Molecular masses are noted at the left in kilodaltons.