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. 2024 Sep 2;56(9):1980–1990. doi: 10.1038/s12276-024-01298-9

Fig. 3. STAT3 promotes NLRP3 inflammasome activation at the transcriptional level.

Fig. 3

a Quantitative PCR analysis of STAT3 mRNA expression in mouse peritoneal macrophages after transfection with NC-siRNA or STAT3-siRNA for 48 h. b IL-1β and TNF-α secretion in supernatants from mouse peritoneal macrophages transfected as described in (a) and then treated with the indicated stimuli. c, d Relative NLRP3 and IL-1β mRNA expression (c) in peritoneal macrophages treated with LPS for 2 h and 4 h or not treated after transfection as described in (a). Target mRNA expression was normalized to the expression of β-actin as the reference gene. Immunoblot analysis of NLRP3 and Pro-IL-1β expression in mouse peritoneal macrophages transfected as described; the photographs are representative of three biologically independent experiments with similar results (d). The results are presented as the means ± SDs; n = 3 biologically independent experiments (a–c). Statistical analyses were carried out via independent sample t-test in (a) or two-way ANOVA with the Bonferroni correction in (b, c). *P < 0.05, **P < 0.01 and ***P < 0.001.