Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Sep 2;56(9):1980–1990. doi: 10.1038/s12276-024-01298-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Immunoblot analysis of cytoplasmic components using a cytoplasmic and nuclear fractionation kit after treatment with the corresponding stimuli described above. b The interaction between NLRP3 and STAT3 and the co-localization with mitochondria (red) in peritoneal macrophages were visualized by a PLA (green) and confocal microscopy. Scale bar: 10 μm. c Cellular thermal shift assay (CETSA) of STAT3 or NLRP3 with napabucasin (20 μM). d LPS-primed peritoneal macrophages were treated with the indicated stimuli. The colocalization of NLRP3 with mitochondria was visualized by immunofluorescence microscopy. Mitochondria were stained with MitoTracker CMXRos, NLRP3 was detected with Alexa Fluor 488, and cellular nuclei were stained with DAPI. The imaging data are representative of several images from three independent experiments. Scale bar: 20 μm. e Immunoprecipitation and immunoblot analysis of lysates from mouse peritoneal macrophages treated with the indicated stimuli with or without napabucasin (10 μM) prior to IP with an anti-STAT3 antibody and immunoblotting with an anti-NLRP3 antibody. f Cytoplasmic components were extracted with a cytoplasmic and nuclear extraction kit after stimulation as described above and were then analyzed by Western blotting. The results are presented as the means ± SDs, and representative photographs of three biologically independent experiments with similar results are shown.