Fig. 6. CPP1 induces glycosaminoglycan accumulation in the abdominal infrarenal aorta.

A Abdominal infrarenal aortic samples were stained with Alcian Blue to visualize glycosaminoglycans. B There was no significant difference in cross-sectional area of the tunica media between CPP1-treated samples and control. C Interestingly, there was a significantly increased amount of Alcian blue positivity in the tunica media of CPP1-treated infrarenal aortic samples. Scale bar = 20 µm. n = 9. C Statistical analysis was performed using a one sample t-test (hypothetical value = 0) on log₂ transformed values. *p < 0.05. Error bars represent standard error of the mean (SEM). CPP1 = Primary calciprotein particle.