TABLE 2.

Priming of CD8⁺ and CD4⁺ T cells and antibodies in mice by HIV gag DNA^a

DNA dose (ng)	% of cells specific for Gag
	CD8+				CD4+
	Saline		PLG		Saline		PLG
	Expt 1	Expt 2	Expt 1	Expt 2	Expt 1	Expt 2	Expt 1	Expt 2
0 (control)	0.1	0.0	nd	nd	0.1	0.3	nd	nd
1	0.1	0.1	7.2	13.9	0.1	0.2	0.9	1.1
10	0.2	0.2	8.4	13.5	0.1	0.3	1.1	0.7
10,000	21.6	24.1	nd	nd	1.9	1.9	nd	nd

^aGroups of 10 CB6 F₁ mice were immunized a single time with the indicated doses of DNA in saline or PLG/CTAB or unimmunized (control). At 28 days, mice were challenged with recombinant vaccinia virus encoding HIV gag, and 5 days later spleens were harvested for analysis of IFN-γ production, as measured by flow cytometry. Data are presented as mean percentages of CD4⁺ or CD8⁺ T cells specific for Gag for two separate experiments, each involving pools of five spleens. nd, not done.