REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-KRT6A, dilution 1:1,000	Proteintech	10590-1-AP
Anti-GAPDH, dilution 1:1,000	Abcam	Ab181602
Anti-wide spectrum cytokeratin antibody, dilution 1:1,000	Abcam	Ab9377
Anti-Ki67, dilution 1:1,000	Abcam	Ab15580
Anti-human ANXA1, dilution 1:1,000	Abcam	Ab214486
Anti-cleaved-caspase 3 (Asp175) antibody, dilution 1:1,000	CST	9661
Anti-mouse IgG, HRP-linked antibody, dilution 1:1,000	CST	7076
Anti-rabbit IgG, HRP-linked antibody, dilution 1:1,000	CST	7074
Biological samples
ESCC tumor samples	Linzhou Cancer Hospital	N/A
Chemicals, peptides, and recombinant proteins
Fetal bovine serum	CELL Technologies	30070
DMEM	Corning	10-013-CV
G418	Sigma	Cat# A1720
Hygromycin B	Sigma	Cat# 400050
Penicillin-Streptomycin	Gibco	Cat# 15140122
Advanced DMEM/F12	Gibco	12634028
RIPA lysis buffer	Beyotime	P0013D
Phosphate-buffered saline (PBS)	TransGen Biotech	FG701
Penicillin/Streptomycin (10,000 U/mL)	TransGen Biotech	FG101
10% Paraformaldehyde fix solution	Beyotime	P0099
Protease and phosphatase inhibitor cocktail	NCM Biotech	P002
Collagenase IV	Gibco	17104019
Hyaluronidase	Sigma-Aldrich	H1115000
DAPI	ZSGB-Bio	ZLI-9557
EDTA	Amresco	97061-022
Matrigel	Corning	356234
DMSO	Sigma	D2650
Hematoxylin	Amresco	0701
Eosin	Amresco	0109
Cryopreservation medium	New Cell & Molecular Biotech	C40100
CryoStor CS10	STEMCELL	07930
Critical commercial assays
PV-9000 immunohistochemistry kit	ZSGB-Bio	PV-9000
DAB chromogenic kit	ZSGB-Bio	ZLI-9017
PANO Multiplex IHC kit (7-color TSA-RM-24259)	PANOVUE	10004100100
Experimental models: Cell lines
L-WRN	ATCC	CRL-3276
Experimental models: Organisms/strains
NSG mouse (female, 6 weeks of age)	IDMO	MPIdKO
Software and algorithms
Prism 8.0	GraphPad Software	http://www.graphpad.com/
ImageJ	NIH	https://imagej.nih.gov/ij/
Other
X-ray irradiation system	Faxitron	MultiRad 225
Falcon 40 μm cell strainer	Corning	352340
50 mL centrifuge tube	Life Sciences	430829
15 mL centrifuge tube	Life Sciences	430791
Dissecting scissors	Sigma-Aldrich	Z265969
12-well cell culture plate	Corning	3512
Primary tissue storage solution	BioGenous	K601005
Surgical suture needles with thread	Beyotime	FS249
Blocking solution	Beyotime	P0102