Esophageal Organoid Culture medium

Reagent	Final concentration	Amount	Identifier
Advanced DMEM/F12	92%	459.3 mL	Gibco, Cat# 12634028
Glutamax (100×)	1×	5 mL	Gibco, Cat# 35050061
HEPES (1 M)	10 mM	5 mL	Gibco, Cat# 15630080
B27 supplement (50×)	1×	10 mL	Gibco, Cat# 17504044
N2 supplement (100×)	1×	5 mL	Gibco, Cat# 17502048
L-WRN conditioned medium	3%	15 mL	N/A
EGF (50 μg/mL)	50 ng/mL	500 μL	Gibco, Cat# PMG8043
Nicotinamide (100 μM)	10 nM	50 μL	Sigma, Cat# 72340
A83–01 (500 mM)	500 μM	50 μL	Tocris Bioscience, Cat# 2939
SB202190 (100 μM)	10 nM	50 μL	Sigma, Cat# S7067
Y27632 (50 μM)	10 nM	50 μL	Selleck Chemicals Cat# S1049
Total	N/A	500 mL

Store at 4°C for up to two months.