Table 8. Results of Placket–Burmann experimental design.
| ObtainedP Values |
R2 Value | |||||
|---|---|---|---|---|---|---|
| Results | Wavelength | Column Temperature | Flow Rate | pH Value of Mobile Phase | Model | Model |
| Carglumic acid assay (%) | 0.415 | 0.652 | 0.771 | 0.951 | 0.512 | 0.7812 |
| Related compound A (%) | 0.598 | 0.142 | 0.598 | 0.028 | 0.116 | 0.6102 |
| Pyrocarglumic acid | 0.879 | 0.197 | 0.454 | 0.008 | 0.054 | 0.6951 |
| Dicarbamoyll-glutamic acid | 1.000 | 0.104 | 0.104 | 0.104 | 0.125 | 0.6000 |
| Pyroglutamic acid | 0.423 | 0.785 | 0.785 | 0.423 | 0.802 | 0.1869 |
| Total impurities | 0.415 | 0.127 | 0.536 | 0.415 | 0.378 | 0.4134 |