Table 1. Regression Equations of HHC in All Matrix, R2, Linear Range, LLOQ, and S/N Ratioa.
| matrix | regression equation | R2 | linear range (ng/mL) | LLOQ (ng/mL) | S/N ratio at LLOQ |
|---|---|---|---|---|---|
| plasma | y = 1.7797 × 10–3x – 1.5662 × 10–3 | 0.9979 | 5–500 | 5.00 | 23.40 |
| liver | y = 1.8441 × 10–3x + 5.7768 × 10–4 | 0.9989 | 16.30 | ||
| kidney | y = 1.8325 × 10–3x – 1.183 × 10–3 | 0.9955 | 13.20 | ||
| brain | y = 1.8497 × 10–3x – 8.725 × 10–4 | 0.9970 | 28.70 |
a
Acceptance criteria of LLOQ: accuracy within 80–120% of the nominal concentration; precision (CV%) ≤ 20%; signal-to-noise ratio (S/N) ≥ 5.